In certain biotechnology processes, cell culture techniques are leveraged to produce and manufacture therapeutic proteins and antibodies.

An efficient and effective Process Analytical Technology (PAT), based on reliable fluid flow sensor measurement, helps monitor cell growth in bioreactors, improves the throughput (amount passing through the process) of protein production and therefore, reduces the cost of the drug.

That’s where Lenterra’s new PAT can have a significant impact.

Learn more about the technology behind Lenterra’s PAT and the advantages it offers Lenterra customers.

Lenterra’s IN-LINE, real-time fluid flow sensor system is the first PAT that provides accurate measurement data – WITHOUT THE NEED TO INTERRUPT THE PROCESS.

PAT SYSTEM OVERVIEW

Technology

  • The probes (DFF – immersion, RealShear mounted flush with the wall)
  • Optical detection principle
  • Temperature compensation
  • 2-D measurements
  • Measurement system – Interrogator

Technology Advantages:

  • Robust; No moving parts, rigid construction
  • Consistent measurements for indefinite periods without service (not achieved in other flow sensing technologies)
  • Operation with no (WSS) or minimal (DFF) intrusion
  • Chemically inert: only outer surface of the pillar is in contact with fluid – made of stainless steel
  • DFF sensor can be applied in ANY flow (powders, multi-phase, chemically aggressive)
  • Fully optical detection, electronics is far away from the probe: no ignition hazard
  • Temperature range: 0 to 200 C currently , restricted by epoxy used for affixing FBGs, can be increased to 500 C or more by fixing FBGs mechanically
  • wide range of sensitivities
  • DFF probe design parameters: pillar outer and inner diameter and length, material (stainless steel or light plastic (e.g. Teflon)
  • For WSS sensor the floating element diameter is added
  • Combination of design parameters makes it possible to fabricate sensors capable of measuring forces as small as 0.05 mN (e.g. shear forces in protein solutions, bio-reactors) and as large as 50 N (forces in HME, HSWG) or more
  • Unprecedented sampling frequency – up to 500 Hz for long term mea-surements (hours, days) – up to 10 kHz for short term measurements (mi-nutes)
  • Measuring not only a global parameter (e.g. viscosity), but the components (e.g. particle impacts)
  • Adds time as another dimension – for example, if we agree that the fluid does not change significantly in 10 seconds, we can accumulate 10000 measurements at 1 kHz sampling rate:
  • Statistically significant distribution – a number of statistical parameters as potential CPPs